Prolong Gold With Dapi

ProLong Diamond Antifade Mountant with DAPI 冷凍 2 mL P36966 ¥8,000 5 × 2 mL P36962 ¥31,200 10 mL P36971 ¥29,100 ProLong Gold Antifade Mountant 室温 （遮光） 2 mL P10144 ¥6,800 5 × 2 mL P36934 ¥29,800 10 mL P36930 ¥26,400 ProLong Gold Antifade Mountant with DAPI 室温 2 mL （遮光） P36941 ¥8,000 5 × 2 mL P36935 ¥31,200 ... Fetch Document

Cheng-Guo Duan And Jian-Kang Zhu
9. ®Drop 6 μL of ProLong Gold Antifade Mountant with DAPI in the center of nuclei area, and cover with a coverslip. Seal the slide with clear nail polish for observation under a microscope. 4 Notes 1. Prepare NEB1 and NEB2 fresh every time needed. Ideally, all reagents and solutions should be freshly prepared before the experiment. 2. ... Document Viewer

BN0518152 Prolong Diamond Flyer 2015 Final - Fisher Scientific
• Available with or without DAPI counterstain Choosing the right antifade reagent Invitrogen ProLong Diamond and Gold Antifade Mountants • Suppress photobleaching and preserve the signals of your fl uorescently labeled target molecules for long-term storage and analysis. Mounted samples can be saved for months with appropriate storage. ... Document Viewer

Antimicrobial Gold Nanoclusters - Pubs.acs.org
For 15 min, and then ProLong® Gold antifade reagent with DAPI was added. Finally, the bacterial cells were observed under epifluorescent microscope, and the cell numbers of SYTOX green stained bacteria and Hoechst 33342 stained bacteria were counted from three images each contained more than 100 bacteria, respectively. ... Fetch Doc

A Novel Role For Primary Cilia In Airway Remodeling
Coverslips were affixed with ProLong Gold plus 4=,6-diamidino-2-phenylindole (DAPI; Life Technologies, Thermo were mounted in ProLong Gold with DAPI and imaged on a Zeiss LSM-700 confocal microscope. Lentiviral knockdown of IFT88 expression in ASMCs. ... Return Doc

Fitchburg, Wisconsin - Wikipedia
Fitchburg is a city in Dane County, Wisconsin, United States. The population was 25,260 at the 2010 census. The population was 25,260 at the 2010 census. Fitchburg is a suburb of Madison and is part of the Madison Metropolitan Statistical Area . ... Read Article

A.$OBJECTIVE$ B.$CAUTIONS$ - Parentprojectmd.org
•!Primary!antibodies!(Before!you!start,!double!check!to!make!sure!the!primary!antibody!is! compatible!with!the!species!of!the!tissue!of!interest!and!the!secondary ... Retrieve Full Source

Immunoflourescent Staining Of Cells For Microscopy
- Alternatively you can use a mounting medium that has DAPI stain (for nuclei) in it. It is also good to prevent bleaching. Prolong Gold Antifade with DAPI (Molecular Probes Cat# 36931) - With a syringe needle shaped as a hook dislodge the laminin coverslip from the bottom of the wells and leave it “standing” on its side. ... Retrieve Document

Clonal Bifurcation Of Foxp3 Expression Visualized In ...
ProLong Gold Antifade Mountant with DAPI (Invitrogen) was used to mountcoverslipsandstainDNA.Cellswerestainedwithprimary Abs for 1 h at room temperature or 4°C overnight, followed by washing in blocking buffer. Secondary Abs were stained for 1 h at ... Return Document

Differentiation Of Primate Primordial Germ Cell-like Cells Following Transplantation Into The Adult Gonadal Niche
Human embryonic stem cells can be differentiated in vitro into primordial germ cell-like cells (PGCLCs) that resemble early primordial germ cells (PGCs). Here the authors transplant PGCLCs generated ... Read News

Table 1 Contents And Storage - Ku
ProLong® Gold and SlowFade® Gold Antifade Reagents | 3 Extended Storage of Samples Mounted with ProLong® Gold Antifade Reagent Following the curing time, the edges of the coverslip can be completely sealed with epoxy or nail polish, and the sample stored at room temperature, at 4°C, or at ≤–20°C. ... Return Doc

SlowFade Antifade Kit - Thermo Fisher Scientific
®SlowFade Antifade Kits 2 with DAPI (P36931, P36935, S36938, S36939). Detailed protocols for SlowFade® Gold and ProLong® Gold reagents are available on our website at probes.invitrogen.com. Materials ... Fetch Document

FISH COFISH Protocol - Titia De Lange
1:750 DAPI from 0.5 mg/ml stock. 7. Dehydrate in ethanol series: 5 min in 70%, 95%, 100% ethanol at rt and air-dry slides. 8. Mount using ProLong Gold, seal with nail polish, store at -20°C protected from light. ... Access Doc

Mounting Media And Antifade Reagents
Prolong is a two-component mounting medium, which begins as an antifade powder and a liquid-base component. The two components are mixed just prior to use. This product has recently been joined by Prolong Gold (which comes premixed but is more expensive per ml.). ... Access Doc

Immunofluorescence (Indirect Staining) Protocol For Adherent ...
Anti-fade mounting medium: ProLong Gold e.g. ntifade A Mountant (Life Technologies, Invitrogen TM , catalog number: ) with DAPI (if nuP10144 clear stainingis needed ) or without DAPI ... Read Document

Activation Of Hypoxia-inducible Factors In Hyperoxia Through ...
Min. After mounting with Prolong Gold-DAPI, slides were viewed by using a Zeiss Axiovert 200 M fluorescent microscope and digital images recorded by using SLIDEBOOK software (In-telligent Imaging Innovations, Denver). VEGF Real-Time PCR. Total RNA was isolated and VEGF mRNA from HLMVE-A and A549 cells was quantified as described (23 ... Fetch Content

FORMALDEHYDE FIXATION AND IMMUNOSTAINING OF CELLS CYTOSPUN OR ...
6. Counterstain DNA with 0.5 - 1X DAPI diluted in TBS-T for 1-2 min. 7. Remove DAPI and add TBS-T. Part 4: Mounting the Coverslips 1. If using SlowFade Gold or ProLong Gold mounting media, remove from the freezer ahead of time and allow to come to room temperature before using. 2. ... View Document

Detection Of Lipids In Embryos By Nile Red Fluorescence
Of ProLong® Gold antifage around the embryo and carefully place a cover slide on top of the drop. o Lipid droplets can be visualized at 200x and 600x using a FITC filter; Nuclei counter-stained with Hoescht (optional) can be examined using a DAPI (UV) filter (UV) Figure 1. ... Return Document

Talk:Operation Barbarossa/Archive 2 - Wikipedia
This is an archive of past discussions. Do not edit the contents of this page. If you wish to start a new discussion or revive an old one, please do so on the current talk page. ... Read Article

BD Horizon Brilliant™ Violet 480 Imaging Reagents
Permeabilization buffers, as well as ProLong® Gold antifade embedding medium, which results in excellent photostability (Figure 4). The BD Horizon Brilliant™ unique polymer dyes were developed from pioneering polymer dye technology acquired from Sirigen Ltd. These dyes deliver more and brighter dye choices over ... View Full Source

Sample Preparation For STED CW 1 And 2-color
• Mount in Prolong Kit (or TDE + antifade). • Let cure at least overnight (at best 48 hrs to reach the maximum RI). • Keep the slide at 4 degree C and protected from the light. ... Access Full Source

Immunofluorescence Author: S. Clouthier Rev: Issued: 6/19 ...
Mount slides with Invitrogen ProLong Gold Antifade Reagent including DAPI (Cat # P-36931) Use the minimum amount necessary for mounting results in better picture quality (about one drop, about 20-30 uL per drop) then carefully add cover slide 3.27 Storage: Store slides overnight in the DARK at room temperature 3.28 Pictures: ... Doc Viewer

Supplementary Information - Media.nature.com
MM PIPES, pH 7.4. Slides were mounted with ProLong® Gold Antifade Reagent with DAPI. DAPI-staining is depicted by a blue fluorescence emission. White arrows indicate extracellular genetic material and the magnified insert shows a close-up of DAPI-stained cell nuclei. Magnification X 400, scale bar: 50 μm. ... Document Viewer